Comparison of activated sludge microbial communities using biolog^TM microplates

Abstract

Laboratory models of wastewater treatment plants (WWTP) provide controlled systems for studying chemical biodegradability and removal as well as WWTP microbial ecology and engineering. In this study, Continuous Activated Sludge (CAS, 3-L) and Semi-Continuous Activated Sludge (SCAS, 2.5-L) units were maintained for up to 17 weeks using feedstocks of either fresh WWTP sewage, a complex synthetic wastewater, or a simple glucose/peptone feed (SCAS only). The goal of this research was to evaluate the microbial communities of the WWTP and the CAS and SCAS units to determine which laboratory models, and which feedstocks, were able to maintain the complexity of the WWTP microbial communities in the laboratory. One endpoint evaluated in this study was microbial community metabolic profiles, as measured using Biolog MicroPlates. Biolog Microplates contain 95 different pre-dried carbon sources and a tetrazolium dye used to spectrophotometrically measure oxidation of carbon sources. The Biolog carbon source utilization patterns of the CAS communities were similar to the SCAS communities when both were fed WWTP sewage. In addition, the profiles for these laboratory models remained similar to the WWTP microbial communities, even over an extended cultivation time (16 weeks) in the CAS systems. Amendment with a complex synthetic wastewater (25% by chemical oxygen demand) did not affect microbial metabolic response in the CAS systems, but amendment (100% by chemical oxygen demand) with a simple glucose-peptone wastewater in the SCAS system resulted in a measurable shift in microbial metabolic response.